Chromium  Agrian Contaminated Water may Cause Testicular Anomalies and Infertility

Dr. Tooba Hafeez Butt (Australia); Tahir Baloch; Dr. Khawja Raees Ahmad; Dr Asmatullah; Tehreem Fatima; Haider Ali Akhtar

Chromium Agrian Contaminated Water may Cause Testicular Anomalies and Infertility

Authors

Dr. Tooba Hafeez Butt (Australia) 4. Australian health, practitioner regulation agency, Unit 62, 15-Vitko Street Woodridge, QLD 4114, Hervey Bay / Bundaberg/Mary borough- Queensland, Australian.
Tahir Baloch Associate Professor, Government College 75 Sargodha, Pakistan
Dr. Khawja Raees Ahmad Professor of Zoology, University of Sargodha, Pakistan.
Dr Asmatullah Professor of Zoology, University of the Punjab, Lahore, Pakistan
Tehreem Fatima M.B.B.S student, Isfahan Medical University, Iran.
Haider Ali Akhtar B.S Zoology, UCP Punjab College, Sargodha, Pakistan

Keywords:

Chromium; , Testicular histopathology; , multi-karyocytes and Seminiferous tubules

Abstract

Objective: Agricultural quantitative products always depend upon soil and water quality. We aim to probe the toxicity of metal-contaminated water used for irrigation in the big cities vicinity of Pakistan. Testicular histopathology of hexavalent chromium (Cr+6) was investigated in adult male albino laboratory mice (Mus musculus). Design: Histopathological experimental study.Place and Duration: The experiment was designed at Sargodha University from 2012 to 2014.Materials and Methods: Six groups (n=15) were given 0.0, 12.5, 25, 50, 100 and 200ppm Cr+6 in drinking water. Five animals from each group were sacrificed at 1, 2, and 3-month stages to recover testes for histopathological studies. Results: A significant (p<0.01) increase in mean cross-sectional area (CSA) of the seminiferous tubules (ST) was noted in 12.5ppm in all durations. CSA of ST elevated in 25 and 50ppm in 1-2nd month but declined in the 3rd month, however, secondarily deteriorated in the 1-3rd month in 100 and 200ppm as compared to control. Daily average water intake and body weight were inverse while chromium intake was directly correlated to Cr+6 concentrations. The mean testicular-somatic index increased in 12.5, 25, and 50ppm groups but decreased in 100 and 200ppm groups at all stages (1-3 months) compared to control. The presence of multi-karyocytes, short-tail spermatozoa, and necrosis of interstitial cells in 50-200ppm groups indicate the signs of infertility. Conclusion: Results indicate that exposure of above 50ppm Cr in water causes various toxicological changes in the testis and such contaminated water should be banned to irrigate the crops and vegetables.

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Chromium Agrian Contaminated Water may Cause Testicular Anomalies and Infertility