Invitro Evaluation of Native Herbal Pesticide against Potato Pest (White Grub) Collected From Skardu District

Ambreen Ali; Ishtiaq Hussain; Muhammad Ayub; Zakir Hussain; Muhammad Ali

Invitro Evaluation of Native Herbal Pesticide against Potato Pest (White Grub) Collected From Skardu District

Authors

Ambreen Ali Researcher, Department of Botany, University of Baltistan Skardu, Pakistan
Ishtiaq Hussain Associate Professor, Department of Botany, University of Baltistan Skardu, Pakistan
Muhammad Ayub Pakistan Agricultural Research Council, Agricultural Research Station, Pakistan
Zakir Hussain Pakistan Agricultural Research Council, Agricultural Research Station, Pakistan
Muhammad Ali Researcher, Department of Botany, University of Baltistan Skardu, Pakistan

Keywords:

Artemisia; , ferula; , bio-pesticidal activity; , white grub; , mortality; , repellency.

Abstract

Farmers in Gilgit-Baltistan are extensively using pesticides to control white grubs, leading to ecological concerns. Field surveys revealed that amount potato tuber damage varied by locality and variety, with the highest infestation (48%) in a non-registered potato variety at Katpana and the lowest (25%) in the Roko variety grown at Sadpara, Skardu. White grubs, also known as chaffer beetles or May-June beetles, are polyphagous pests that damage a wide range of crops. The present study evaluated the pesticidal effects of ethanol-based extracts from Artemisia sieversiana and Ferula jieschekiana under controlled conditions. Results revealed statistically significant (p≤0.05) among different concentrations of selected species. Bioassays were conducted using three concentrations (1%, 3%, and 5%) of dried plant parts. The highest mortality (90%) was recorded with 5% A. sieversiana after 72 hours, while F. jieschekiana at 5% showed 80% mortality. Repellency tests indicated maximum repellency (50%) in F. jieschekiana after 90 minutes. Pellets made from F. jieschekiana retained shape better and were more effective in repellency after 90 minutes compared to A. sieversiana. Overall, both species showed promising mortality and repellency effects, highlighting their potential as eco-friendly bio-pesticide alternatives to harmful chemical insecticides for sustainable pest management in potato farming.

References

Abbas, Z., Alam, J., Khan, S. M., Hussain, M., & Abbasi, A. M. (2019). Diversity, ecological feature and conservation of a high montane flora of the Shigar valley (Karakorum Range) Baltistan region, northern Pakistan. Pak. J. Bot, 51(3), 985-1000.

Abbott, W.S. (1925) A method of computing the effectiveness of an insecticide, J. Econ. Entomol. 18: 265-267.

Akutse, K. S., Subramanian, S., Maniania, N., Dubois, T., & Ekesi, S. (2020). Biopesticide research and product development in Africa for sustainable agriculture and food security–Experiences from the International Centre of Insect Physiology and Ecology (icipe). Frontiers in Sustainable Food Systems, 152.

Ayub, M., Hussain, S., Rasool, A., Hussain, I., Khan, F., & Sultan, A. (2019). Insecticidal activity of Artemisia sieversiana Ehrh. plant extract against white grub (Scarabaeidae: Coleoptera). J. Entomo. Zool. Studies, 7, 304-308.

Chandal RS, Pathania M, Verna KS, Bhatacharyya B, Vashishth S and Kumar V. 2015. The ecology and control of potato white grubs of India. Potato Res. 58:147-164.

Hadizadeh, I., Peivastegan, B., Hannukkala, A., Van der Wolf, J. M., Nissinen, R., & Pirhonen, M. (2019). Biological control of potato soft rot caused by Dickeya solani and the survival of bacterial antagonists under cold storage conditions. Plant pathology, 68(2), 297-311.

Hayat, M. Q., Khan, M. A., Ashraf, M., & Jabeen, S. (2009). Ethnobotany of the genus Artemisia L. (Asteraceae) in Pakistan. Ethnobotany Research and Applications, 7, 147-162.

Hussain, I., Bano, A., & Ullah, F. (2011). Traditional drug therapies from various medicinal plants of central karakoram national park, Gilgit-Baltistan Pakistan. Pak J Bot, 43, 79-84.

Hussain, Z., Tahir, M. M., Rahim, N., Khaliq, A., Facho, Z. H., Shafqat, H., ... & Shaheen, A. (2019). 23. Fertility assessment of mountainous soils of District Skardu, Gilgit-Baltistan, Pakistan. Pure and Applied Biology (PAB), 8(3), 2095-2103.

Kumar, K. K., Sridhar, J., Murali-Baskaran, R. K., Senthil-Nathan, S., Kaushal, P., Dara, S. K., & Arthurs, S. (2019). Microbial biopesticides for insect pest management in India: Current status and future prospects. Journal of Invertebrate Pathology, 165, 74-81.

Kumar, S., & Pandey, A. K. (2022). Population density of white grubs (Scarabaeidae: Coleoptera) in different crop ecosystem.

Laub, C. A., Youngman, R. R., Jordan, T. A., & Kuhar, T. P. (2018). Compact soil sampling strategy for white grubs.

Marrone, P. G. (2014). The market and potential for biopesticides. In Biopesticides: state of the art and future opportunities (pp. 245-258). American Chemical Society.

Mazid M. T.A. Khan, Z. H. Khan, S. Quddusi and F. Muhammad, 2011. Occurance, biosynthesis and potentialities of ascorbic asid in plants. International journal of plant, animal and environmental sciences.

Motai, T., Daikonya, A., & Kitanaka, S. (2004). Sesquiterpene coumarins from Ferula fukanensis and nitric oxide production inhibitory effects. Journal of natural Products, 67(3), 432-436.

Niber, B. T. (1994). The ability of powders and slurries from ten plant species to protect stored grain from attack by Prostephanus truncatus Horn (Coleoptera: Bostrichidae) and Sitophilus oryzae L.(Coleoptera: Curculionidae). Journal of Stored Products Research, 30(4), 297-301.

Nosheen, A., Yasmin, H., Naz, R., Bano, A., Keyani, R., & Hussain, I. (2018). Pseudomonas putida improved soil enzyme activity and growth of kasumbha under low input of mineral fertilizers. Soil Science and Plant Nutrition, 64(4), 520-525.

RAHMAN, S., BISWAS, S. K., BARMAN, N. C., & FERDOUS, T. (2016). Plant extract as selective pesticide for integrated pest management. Biotechnological research, 2(1), 6-10.

Sahebkar, A., & Iranshahi, M. (2010). Biological activities of essential oils from the genus Ferula (Apiaceae). Asian Biomedicine, 4(6), 835-847.

Samada, L. H., & Tambunan, U. S. F. (2020). Biopesticides as promising alternatives to chemical pesticides: A review of their current and future status. Online J. Biol. Sci, 20(2), 66-76.

Skendžić, S., Zovko, M., Pajač Živković, I., Lešić, V., & Lemić, D. (2021). Effect of climate change on introduced and native agricultural invasive insect pests in Europe. Insects, 12(11), 985.

Theurkar, S. V. (2014). Investigation of the effects of two different biopesticides on the mortality of Holotrichia serrata F. adults (Coleoptera: Scarabaiedae). International Journal of Pharma Sciences and Research, 5, 397-401.

Yaqoob, U., & Nawchoo, I. A. (2017). Impact of habitat variability and altitude on growth dynamics and reproductive allocation in Ferula jaeschkeana Vatke. Journal of King Saud University-Science, 29(1), 19-27.

Zaki, F. A., Wani, Z. H., & Wani, A. R. (2006). Relative efficacy of various insecticides and bio-pesticides against white grub damaging potato under rainfed conditions. Applied Biological Research, 8(1and2), 51-54.

Invitro Evaluation of Native Herbal Pesticide against Potato Pest (White Grub) Collected From Skardu District