Boosting Growth and Immunity in Fish Variety Rohu Fingerlings (Labeorohita): The Role of Bacillus Subtilis as a Probiotic in Semi-Intensive Aquaculture

Muhammad Kashif Hameed; Waqas Ameen; Tabay Hassan; Mukhtar Ahmad; Asma Naqia

Boosting Growth and Immunity in Fish Variety Rohu Fingerlings (Labeorohita): The Role of Bacillus Subtilis as a Probiotic in Semi-Intensive Aquaculture

Authors

Muhammad Kashif Hameed MS Student, Department of Zoology, Punjab University, Lahore, Punjab, Pakistan
Waqas Ameen MS Student, Department of Zoology, Islamia University of Bahawalpur, Punjab, Pakistan https://orcid.org/0009-0006-6063-7400
Tabay Hassan MS Student, Department of Zoology, Islamia University of Bahawalpur, Punjab, Pakistan
Mukhtar Ahmad MS Student, Department of Zoology, Islamia University of Bahawalpur, Punjab, Pakistan
Asma Naqia MS Student, Department of Zoology, Islamia University of Bahawalpur, Punjab, Pakistan https://orcid.org/0009-0005-9616-250X

Keywords:

Labeo rohita, Boosting Growth, Bacillus subtilis, Hematology, Semi-Intensive Aquaculture

Abstract

Aquaculture plays a significant role in rapidly increasing fish production to meet the world's growing protein needs. The goal of the current study was to determine the response of Indian major carp Rohu fingerlings (Labeo rohita) in terms of growth, Haematological, and immune parameters after the dietary supplementation with probiotic Bacillus subtilis. An 8-week feeding trial was carried out under a semi-intensive culture system. During the trial, fish were fed with three diets, including a control diet and two experimental diets with varying concentrations of probiotics. Probiotic concentrations in experimental diets were 103 and 106 CFU g-1, respectively. All these treatments were applied in triplicate. After the experiment, the growth, hematological, and immune parameters of the fish were evaluated. One-way ANOVA followed by post hoc LSD Tukey test based results indicated that growth rate, weight gain, and specific growth rate were significantly (P<0.5) higher in fish fed with a probiotic-containing diet, with the highest growth observed with a diet containing 106 CFU/g of probiotic. Leucocyte, erythrocyte count, and glucose content were also high in experimental groups as compared to control groups. Total protein content and Peroxidase activity were significantly higher (P<0.5) in fish fed with the highest concentration (106 CFU/g) of probiotic as compared to the control and other dietary groups. From these outcomes, Bacillus subtilis can be concluded as a potential probiotic for L. rohita culture under a semi-intensive culturing system and can be used for better growth and survival rate of fish in aquaculture practices.

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Boosting Growth and Immunity in Fish Variety Rohu Fingerlings (Labeorohita): The Role of Bacillus Subtilis as a Probiotic in Semi-Intensive Aquaculture