Influence of Grinding-Induced Particle Size Reduction on the Physicochemical, Functional, and Sensory Properties of Moringa oleifera Leaf Powder
DOI:
https://doi.org/10.67244/jai.bwo-researches.v4i3.a248Keywords:
Moringa oleifera, particle size distribution, grinding methods, nutritional quality, functional properties, sensory evaluationAbstract
Moringa oleifera is widely valued for its nutritional potential, yet the particle size of its leaf powder critically influences its physicochemical and functional properties. This study evaluated the effect of different particle sizes, obtained through varying grinding intensities, at Sindh Agriculture University, Tandojam. Fresh leaves were dehydrated at 40–45°C and subjected to three treatments: T₁ (pestle), T₂ (blender), and T₃ (grinding machine). The Physicochemical analyses were conducted according to AOAC (2016) methods, while functional and sensory properties were evaluated using standard analytical procedures. Alongside functional properties including water absorption capacity (WAC), oil holding capacity (OHC), particle size distribution, pH, yield, and weight loss variation, all the treatments were conducted in three replications. Results revealed significant (P < 0.05) differences among treatments. T₃ obtained the finest particles (20, 40, 60, 80, and 120 µm), the highest values of ash (9.02%), protein (21.19%), carbohydrates (35.71%), WAC (4.6g/g), and OHC (3.1g/g). Median values were observed in T₁ and T₂, with T₂ generally exceeding T₁ in nutritional and functional properties. Yield was lower in ground samples (30.25–31.31%) due to fine particle losses, while post-drying weight decreased progressively from T₁ (22.52) to T₃ (20.61 g). Sensory evaluation confirmed the influence of grinding intensity, with T₃ recording the highest scores (7.66) for all attributes, followed by T₂ and T₁. These results highlight superior consumer preference for the finely ground powder, supported by significant differences (P< 0.05) across treatments. These findings demonstrate that reducing particle size through grinding machines significantly enhances the physicochemical, nutritional, and functional properties of Moringa oleifera leaf powder (MOLP), making it highly suitable for functional foods, dietary supplements, and therapeutic formulations. Future studies should optimize grinding parameters for industrial scalability.
References
Al-Mamun, M. A., & Hossain, M. M. (2022). Effects of particle size on storage stability and quality attributes of dehydrated vegetable powders. Journal of Food Storage and Preservation, 48(3), e16892.
Ali, M., Ahmed, M., & Hussain, T. (2017). Effect of grinding methods on the yield and quality of plant-based powders. Pakistan Journal of Food Sciences, 27(3), 112-119.
Amaglo, N. K., & Afolabi, I. S. (2020). Nutritional and medicinal value of Moringa leaves: A review. Food Chemistry, 100(1), 243-250.
Armel, A. A. J., Edith, F. N., & Moses, M. C. (2021). Influence of compound contents and particle size on some functional properties of Moringa oleifera leaves (Lam) powders. Asian Food Science Journal, 20(1), 60-71.
Astrini, N. J., Rakhmawati, T., Sumaedi, S., & Bakti, I. G. M. Y. (2020). Identifying objective quality attributes of functional foods. Quality Assurance and Safety of Crops & Foods, 12(2), 24-39.
Bai, Y., Wu, X., Gu, J., Zhang, R., & Zhang, H. (2020). Influence of grinding methods on the properties of Moringa oleifera leaf powder. Journal of Food Science and Technology, 57(1), 250-258.
Barretto, R., Buenavista, R. M., Pandiselvam, R., & Siliveru, K. (2022). Influence of milling methods on the flow properties of ivory teff flour. Journal of Texture Studies, 53(6), 820-833.
Bashir, K., Ali, S., & Bafakeeh, O. T. (2018). Moringa oleifera: A comprehensive review on its pharmacological properties. Journal of Evidence-Based Integrative Medicine, 23(2), 1-10.
Bashir, K., Ali, S., & Bafakeeh, O. T. (2018). Moringa oleifera: A comprehensive review on its pharmacological properties. *Journal of Evidence-Based Integrative Medicine, 23*, 1-10.
Bhat, K. R., Ravi, B. S., & Prakash, R. M. (2023). Antimicrobial activity of Moringa oleifera. International Journal of Pharmacognosy and Phytochemical Research, 2(3), 145-148.
Chopra, P., Ghosh, A., & Kaur, C. (2022). Impact of particle size on the physicochemical properties of Moringa leaf powder. Journal of Food Processing and Preservation, 46(1), e16631.
Dhiman, A., Suhag, R., & Thakur, D. (2021). Impact of particle size reduction on bioavailability of phytochemicals from plant-based foods: A review. Critical Reviews in Food Science and Nutrition, 61(22), 3741-3758.
Fahey, J. W., Wade, K. L., Stephenson, K. K., Shi, Y., Liu, H., Panjwani, A. A., ... & Olson, M. E. (2019). A strategy to deliver precise oral doses of the glucosinolates or isothiocyanates from Moringa oleifera leaves for use in clinical studies. Nutrients, 11(7), 1547.
Fidyasari, A., Estiasih, T., Wulan, S. N., & Khatib, A. (2024). The physicochemical, functional, and pasting properties of Moringa oleifera leaf powder from different leaf stalk colors. CyTA-Journal of Food, 22(1), 2402062.
Gharibzahedi, S. M. T., Ansarifard, I., Hasanabadi, Y. S., Ghahderijani, M., & Yousefi, R. (2013). Physicochemical properties of Moringa peregrina seed and its oil. Quality Assurance and Safety of Crops & Foods, 5(4), 303-309.
González-Sarrías, A., & Espín, J. C. (2020). Bioavailability of phenolic compounds in functional foods: Challenges and perspectives. Trends in Food Science & Technology, 96, 174-186.
Gopalakrishnan, L., Doriya, K., & Kumar, D. S. (2016). Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, 5(2), 49-56.
Guevara, A. P., De Dios, R. R., & Ochoa, A. (2021). Evaluation of the hypoglycaemic effect of Moringa oleifera leaves in normal and diabetic rabbits. Journal of Ethnopharmacology, 68(1-3), 117-121.
Gupta, V. K., Chaudhary, N., & Kumar, D. (2021). Nutritional and pharmacological properties of Moringa oleifera: A review. Nutrition Reviews, 79(5), 567-577.
Handayani, Y., Aminah, S., & Yanis, M. (2022, May). Characteristics of Moringa leaf powder as fortification and consumer acceptance. In IOP Conference Series: Earth and Environmental Science (Vol. 1027, No. 1, Article 012005). IOP Publishing.
Huang, X., Liang, K. H., Liu, Q., Qiu, J., Wang, J., & Zhu, H. (2020). Superfine grinding affects physicochemical, thermal and structural properties of Moringa oleifera leaf powders. Industrial Crops and Products, 151, 112472.
Kannangara, P. T., Ratnayake, R. H. M. K., Jayawardana, R. J. M. C. N. K., Marage, H. M. C. K. H., & Sarananda, K. H. (2018). Sensory attributes and shelf-life evaluation of an instant soup powder fortified with Moringa (Moringa oleifera Lam.) leaves. Journal of Food and Agriculture, 11(2).
Lawless, H. T., & Heymann, H. (2019). Sensory Evaluation of Food: Principles and Practices (2nd ed.).
Springer Makkar, H. P. S., & Siddhuraju, P. (2018). Medicinal and nutritional value of Moringa leaves. In S. Kumar & N. Singh (Eds.), Moringa: A sustainable crop (pp. 103-128).
Mishra, A., Yadav, A., & Joshi, P. (2018). Utilization of Moringa oleifera leaves in food processing: A review. Critical Reviews in Food Science and Nutrition, 58(5), 773-785.
Nouman, W., Siddiqui, M. T., & Basra, S. M. A. (2016). Phenolics, antioxidant activity and mineral composition of Moringa oleifera leaves as influenced by harvest time and geographical location. International Journal of Agriculture and Biology, *18*(4), 753-758.
Rojas, M. L., & Augusto, P. E. D. (2020). Milling processes and their influence on the physicochemical properties of plant-based foods: A review. Food Research International, *137*, 109655.
Sakr, H., Ammar, A., Zaki, H., Salama, M. A., & Ali, M. (2024). Impact of ball milling on physicochemical, structural, and functional properties of Moringa oleifera L. leaf powders. Journal of Food Measurement and Characterization, 18(1), 320-330.
Salama, M. A., El-Sharnouby, G., & Nassar, A. (2022). Biological, physicochemical and sensory evaluation of hard biscuit enriched with a powder of moringa leaves (Moringa oleifera L.). Archives of Agriculture Sciences Journal, 5(3), 62-83.
Sanjay, K. V., Tiwari, A., & Singh, D. (2022). Particle size analysis of powdered Moringa leaves: Implications for bioavailability and health benefits. Journal of Nutritional Science, 11, e2.
Sharma, K. D., Karki, S., Thakur, N. S., & Attri, S. (2012). Chemical composition, functional properties and processing of carrot-a review. Journal of Food Science and Technology, 49(1), 22-32.
Siddhuraju, P., & Becker, K. (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of Agricultural and Food Chemistry, 51(8), 2144–2155.
Sugahara, S., Chiyo, A., Fukuoka, K., Ueda, Y., Tokunaga, Y., Nishida, Y. & Yasuda, S. (2018). Unique antioxidant effects of herbal leaf tea and stem tea from Moringa oleifera L., especially on superoxide anion radical generation systems. Bioscience, Biotechnology, and Biochemistry, 82(11), 1973-1984.
Sultana, S. (2020). Nutritional and functional properties of Moringa oleifera. Metabolism Open, 8, 100061.
Sun, X., Zhang, Y., Li, J., Aslam, N., Sun, H., Zhao, J., & He, S. (2019). Effects of particle size on physicochemical and functional properties of superfine black kidney bean (Phaseolus vulgaris L.) powder. PeerJ, 7, e6369.
Tafu, N. N., & Jideani, V. A. (2022). Proximate, elemental, and functional properties of novel solid dispersions of Moringa oleifera leaf powder. Molecules, 27(15), 4935.
Vainio, A., Hakala, J., & Ghosh, A. (2018). Moringa oleifera as a potential source of protein.
Food & Function, 7(9), 3962-3971.
Xiao, W., Zhang, Y., Fan, C., & Han, L. (2017). A method for producing superfine black tea powder with enhanced infusion and dispersion property. Food Chemistry, 214, 242-247.
Xing, Y., Xu, Q., Li, X., Chen, C., Ma, L., Li, S., & Lin, H. (2016). Chitosan-based coating with antimicrobial agents: Preparation, property, mechanism, and application effectiveness on fruits and vegetables. International Journal of Polymer Science, 2016, Article 4851730.
Yadav, M., Lata, S., & Sharma, N. (2019). Nutritional and functional properties of Moringa oleifera. Journal of Food Science and Technology, 53(7), 2876-2884.
Yang, S. L., Yang, R. C., Zhou, X., Yang, S. H., Luo, L. L., Zhu, Y. C., & Boonanuntan, S. (2020). Effects of feeding diets with processed Moringa oleifera stem meal on growth and laying performance, and immunological and antioxidant activities in laying ducks. Poultry Science, 99(7), 3445-3451.
Zaiter, A., Becker, L., Karam, M. C., & Dicko, A. (2016). Effect of particle size on antioxidant activity and catechin content of green tea powders. Journal of Food Science and Technology, 53, 2025-2032.
Zhang, Y., Liu, D., & Guo, Y. (2022). The influence of particle size on the antioxidant activity of Moringa oleifera leaf powder: Implications for food applications. Food Chemistry, 366.
Zhang, H., & Chen, X. D. (2021). Understanding powder adhesion in food processing equipment: Mechanisms and mitigation strategies. Journal of Food Engineering, 290, 110242.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 International Journal of Agriculture Innovations and Cutting-Edge Research (HEC Recognised)

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
BWO Research International
Pakistan