Method Development and Validation for the Determination of Clodinafop-propargyl (EC/WP Formulation) by HPLC-UV-Visible Technique
Keywords:
Clodinafop-propargyl, Development, Validation, Method, HPLCAbstract
Wheat (Triticum aestivum L.) is a major staple food in Pakistan and contributes about 1.6% to GDP. In Pakistan, the area under wheat is approximately 9.16 million hectares, with an overall annual production of 27.46 million tons. It meets the main dietary requirements and feeds about 220.8 million people in the country. The infestation of different weeds is a serious problem for wheat crop production in Pakistan. Generally, it causes a yield loss of about 40% to 60%.
Background: Herbicide samples are tested to determine their active ingredients (a.i.), but various methods yield different results. Thus, the main purpose of the current study was to develop and validate a Clodinafop-propargyl determination method by using the HPLC-UV-Visible technique.
Methods: The HPLC-UV-Visible technique used for the quantification of Clodinafop-propargyl contents in the herbicide sample was validated in the Pesticide Quality Control Laboratory (ISO/IEC: 17025), Bahawalpur. This method validation process includes: Use of Blank, Use of Reference Standard, Specificity, repeatability, reproducibility, Detection Limit (LOD), Quantification Limit (LOQ), Linearity, Measurement Uncertainty, Ruggedness and recovery.
The detection and quantification limits of the current method were 2.4 and 8.0ppm of Clodinafop-propargyl, respectively. The repeatability, however, was 0.1547 %, and the reproducibility (calculated T- was 0.653 thar was less than T-tabulated (i.e., 2.262). The Linear curve was found for the concentration ranging from 200 to 1000 ppm Clodinafop-propargyl, showing R2 of 1. As for recovery is concerned, it was 100.030% for Clodinafop-propargyl in the herbicide sample. The Clodinafop-propargyl concentration was detected with complete recovery without any interference from other materials in the sample. The Z-scores for all PT samples were within an acceptable range. The coefficient of correlation (1%) exhibited a strong correlation between actual Clodinafop-propargyl contents and observed contents.
Conclusions: Based on the above findings, it is assumed that the performance of the method under study was excellent. Therefore, this method can be effectively employed for Clodinafop-propargyl estimation in herbicides.
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