Micropropagation and callus induction in pureline cultivars of tomato (solanum lycopersicum l.)

Rashida  Bibi; Khatir Ali; Farzana Zahid; Saif Ul Islam; Sadia Zahid

Micropropagation and callus induction in pureline cultivars of tomato (solanum lycopersicum l.)

Authors

Rashida Bibi Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad, Pakistan,
Khatir Ali Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad, Pakistan,
Farzana Zahid Agriculture research officer, Agriculture research Wing Lasbela, Pakistan,
Saif Ul Islam Lasbela University of Agriculture Water and Marine Sciences, Lasbela, Pakistan.
Sadia Zahid Department of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture Faisalabad, Pakistan,

Keywords:

America, , tomato, , nutrient, , Antioxidants, , beta-carotene, , fibre, lutein, , lycopene, , vitamins, , regeneration.

Abstract

Tomato (Solanum lycopersicum L.) is a significant vegetable crop as it has both nutritional and economic importance on a global scale. Tomato breeding programs must be able to multiply and improve genetically and through biotechnology through efficient systems of in vitro regeneration. The present study aimed to streamline micropropagation and callus induction procedures with two pure-line tomatoes (BL-1174 and Tinto) with varying auxins and cytokinin combinations. The study involved two experiments that were designed using a completely randomized design (CRD): one was aimed at testing the regeneration of shoots and roots in an experiment using nodal explants, and the other aimed at callus induction with leaf disc explants. Regeneration was undertaken in Murashige and Skoog (MS) medium supplemented with different concentration levels of 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA), whereas 2,4-D and BAP were used in callogenesis. Analysis of variance (ANOVA) was used in data analysis, and the means of treatment were compared with the help of the Tukey HSD test. Treatments and genotypes had significant differences in all the parameters that were evaluated. Tinto pure line had better in vitro performance with the highest induction of shoot (74.3%), shoot length (2.7 cm), root induction (71.7%), number of leaves and roots per explant, survival percentage (68%), and callus induction (90) with increased concentration of BAP and IBA. Internodal distance was found to be relatively higher in BL-1174, and the total regeneration efficiency was lower. The modified hormonal ratios exhibited high genotype responses, as Tinto was more responsive to in vitro regeneration and formation of callus. The protocol developed is a reliable and reproducible system to consider large-scale micropropagation and as a basis for genetic transformation, mutation breeding, and other high biological applications of tomato.

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Micropropagation and callus induction in pureline cultivars of tomato (solanum lycopersicum l.)